美国Applied Biosystems™ 7500实时荧光定量PCR系统
美国Applied Biosystems™ 7500实时荧光定量PCR系统 | 时间: December-25 14:54:34 | 分类:其它仪器
凌仪生物专业提供高速离心机，美国Bio-rad伯乐电泳仪，德国eppendorf艾本德移液器，PCR基因扩增仪，雅培原位杂交仪，Thermo热电离心机等实验室仪器设备，要省钱，别错过哦。 
7500实时荧光定量PCR系统描述
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
NEW - 7500 Software v2.0
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.
The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results
21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow
Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
NEW - 7500 Software v2.0
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.
The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results
21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow
Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
► 功能强大的五色平台已经过校准，支持较多种类的染料：FAM™/SYBR® Green I、VIC®/JOE™、ED™/TAMRA™/Cy®3、ROX™/Texas Red® 和 Cy®5 染料Applied Biosystems® 7500 实时荧光定量 PCR 系统为要求较大的染料功能多样性的实验室提供了一种可靠的平台。该平台具有灵敏的光学系统，适用于宽广光谱范围内的多种荧光染料。可变的激发波长可提高长波长 (红色) 染料的检测灵敏度。
7500 软件
7500 软件融合了多种易于使用的特性，如各种反应板设置向导、标准曲线稀释和预混液配方计算器、QC标记、数据过滤器，并可在运行完成时通过电子邮件通知。7500 软件还包括功能强大的基因表达研究软件包，并提供各种熔解曲线实验方案选项，包括多峰检测、步进式温度控制和可定制的变温速率。可提供遵循美国联邦法规第 21 章第 11 节 (21 CFR Part 11) 的模块SDS v1.4 21CFR Part 11 模块是一款功能强大的工具，它遵循美国联邦法规第 21 章第 11 节的要求，同时还可提供用户可定制的配置设置灵活性。
支持多种应用
其应用领域包括基因表达分析、病原体定量、SNP 基因分型、拷贝数变异、恒温和采用内部阳性对照的 +/–Assay。为方便上述应用领域，Life Technologies 提供了预先配制、已经过质量测试的即用型 TaqMan® Assay，可与 7500 系统配合使用。现在您即可节省在分析优化方面的精力。
升级至 7500 Fast 系统
您可以选择升级至 7500 Fast 系统。该 7500 Fast 系统采用了我们的预混液配方，使您可以将实时荧光定量 PCR 程序缩短至 30 分钟。
7500 系统包括：
► 可选择 Dell® 商用系列笔记本或配有平板显示器的台式电脑
► 化学安装试剂盒包括染料校正反应板、RNA酶 P 仪器验证反应板和应用特异的 starter kit
► 仪器操作和分析软件，以及 Primer Express®引物和探针设计软件
► 包括入门指南在内的丰富的文件集，适用于各种主要的实时荧光定量 PCR 应用领域
► 由 Life Technologies 服务工程师提供的安装服务
7500 软件
7500 软件融合了多种易于使用的特性，如各种反应板设置向导、标准曲线稀释和预混液配方计算器、QC标记、数据过滤器，并可在运行完成时通过电子邮件通知。7500 软件还包括功能强大的基因表达研究软件包，并提供各种熔解曲线实验方案选项，包括多峰检测、步进式温度控制和可定制的变温速率。可提供遵循美国联邦法规第 21 章第 11 节 (21 CFR Part 11) 的模块SDS v1.4 21CFR Part 11 模块是一款功能强大的工具，它遵循美国联邦法规第 21 章第 11 节的要求，同时还可提供用户可定制的配置设置灵活性。
支持多种应用
其应用领域包括基因表达分析、病原体定量、SNP 基因分型、拷贝数变异、恒温和采用内部阳性对照的 +/–Assay。为方便上述应用领域，Life Technologies 提供了预先配制、已经过质量测试的即用型 TaqMan® Assay，可与 7500 系统配合使用。现在您即可节省在分析优化方面的精力。
升级至 7500 Fast 系统
您可以选择升级至 7500 Fast 系统。该 7500 Fast 系统采用了我们的预混液配方，使您可以将实时荧光定量 PCR 程序缩短至 30 分钟。
7500 系统包括：
► 可选择 Dell® 商用系列笔记本或配有平板显示器的台式电脑
► 化学安装试剂盒包括染料校正反应板、RNA酶 P 仪器验证反应板和应用特异的 starter kit
► 仪器操作和分析软件，以及 Primer Express®引物和探针设计软件
► 包括入门指南在内的丰富的文件集，适用于各种主要的实时荧光定量 PCR 应用领域
► 由 Life Technologies 服务工程师提供的安装服务
For Research Use Only. Not for use in diagnostic procedures.
7500实时荧光定量PCR系统规格参数：
| Format: | 96-well plate |
| Optics: | Tungsten-halogen lamp, 5 emission filters, CCD Camera, 5 excitation filters |
| Weight: | 75 lbs |
| Precision: | 99.7% confidence (5000 & 10000 copies) |
| Dimensions: | Width: 34 cm (13.39 in), Height: 49 cm (19.29 in), Depth: 45 cm (17.72 in) |
| Throughput: | 4 to 5-96-well plates per 8-hr day |
| Power Limit: | 950 W |
| Sensitivity: | 1 copy of RNase P gene (human genomic DNA) |
| Product Size: | 1 system |
| Dynamic Range: | 9 logs |
| Input Voltage: | 120V |
| Calibrated Dye: | SYBR® Green I, Cy3™, Cy5™, FAM™, JOE™, VIC™, Texas Red®, TAMRA™, ROX™, NED™ |
| Reaction Speed: | Standard |
| Sample Ramp Rate: | ± 1.1°C⁄sec |
| External Computer: | Notebook: Intel Core 2 Duo T5500 (1.66 GHz), 80 GB HD, 8X DVD, 1.0 G DDR2-533 MHz SDRAM |
| Peak Block Ramp Rate: | 2.5°C⁄sec |
| Temperature Accuracy: | 0.25°C (35°C to 95°C after 3 min) |
| Passive Reference Dye: | ROX (Separate Tube), No ROX, ROX (Pre-mixed) |
| Reaction Volume Range: | 20-100 µl |
| Software Compatibility: | Primer Express, 7500 System Software (RQ included), 21 CFR Part 11 Module (optional) |
| Temperature Uniformity: | 0.50°C (after 30 sec) |
| Thermal Cycling System: | Peltier-Based System |
| For Use With (Equipment): | 7500 System |
| Temperature Range (Metric): | 4-100°C |
| High Throughput Compatibility: | Multiplexing |
7500 实时荧光定量PCR系统订购信息
| 产品名称 | 订货编号 | 数量单位 |
| 7500 Fast System Upgrade Kit | 4362143 | 1 台仪器 |
| 7500 Real-Time PCR System with Dell™ Notebook | 4351104 | 1 台仪器 |
| 7500 Real-Time PCR System with Dell™ Tower | 4351105 | 1 台仪器 |
| Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems | 4359286 | 1 套 |
| Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems | 4359284 | 1 套 |
| 7500 System SDS v1.4 21 CFR Part 11 Module | 4377354 | 1 套 |
7500荧光定量PCR仪订购信息
| 产品描述 | 货号 | 数量 |
| 7500实时荧光定量PCR扩增仪（配备笔记本电脑） | 4351104 | 1台 |
| 7500实时荧光定量PCR扩增仪（配备台式电脑） | 4351105 | 1台 |
| 7500实时荧光定量PCR扩增仪快速模式升级 | 4362143 | 1个 |
| 7500实时荧光定量PCR扩增仪SDS软件21 CFR Part 11 模块 | 4377354 | 1个 |
7500Fast荧光定量PCR仪订购信息
| 产品描述 | 货号 | 数量 |
| 新型高分辨率熔解曲线(HRM)软件 | 4397808 | 1套 |
| 7500快速实时荧光定量PCR扩增仪（配备笔记本电脑） | 4351106 | 1台 |
| 7500快速实时荧光定量PCR扩增仪（配备台式电脑） | 4351107 | 1台 |
| 7500实时荧光定量PCR扩增仪快速模式升级 | 4362143 | 1台 |
| 7500快速实时荧光定量PCR扩增仪SDS软件21 CFR Part 11 模块 | 4377355 | 1套 |
